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random hexamers  (Qiagen)


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    Structured Review

    Qiagen random hexamers
    Random Hexamers, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 2503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexamers/Random+Hexamers/pm42121917-112-10-23
    Average 97 stars, based on 2503 article reviews
    random hexamers - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: Extracellular vesicles carrying surface-anchored adiponectin prevent obesity-related metabolic complications by enhancing insulin sensitivity
    Article Snippet: Liver and muscle RNA were extracted using the Maxwell® RSC simplyRNA Tissue Kit and instrument (Promega). .. RNA quantity and purity were assessed via NanoDrop ND-2000 (Thermo Fisher). cDNA was synthesized from 1 μg RNA using SuperScriptTM II (Invitrogen) and random hexamers, then purified with the Qiaquick PCR kit (Qiagen). qPCR was performed with 3 ng cDNA, MaximaTM SYBR Green Master Mix (Thermo Fisher), and 0.3 μM primers using a CFX Opus 384 system (Bio-Rad). ..

    Article Title: Mitochondrial damage drives T-cell immunometabolic paralysis after major surgery.
    Article Snippet: Quantity and purity of RNA were assessed on a NanoDrop2000 spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA was synthesized with equal amounts of RNA using a Superscript III reverse transcriptase (Invitrogen), oligo (dT) primers and random hexamers (Qiagen). ..

    Purification:

    Article Title: Extracellular vesicles carrying surface-anchored adiponectin prevent obesity-related metabolic complications by enhancing insulin sensitivity
    Article Snippet: Liver and muscle RNA were extracted using the Maxwell® RSC simplyRNA Tissue Kit and instrument (Promega). .. RNA quantity and purity were assessed via NanoDrop ND-2000 (Thermo Fisher). cDNA was synthesized from 1 μg RNA using SuperScriptTM II (Invitrogen) and random hexamers, then purified with the Qiaquick PCR kit (Qiagen). qPCR was performed with 3 ng cDNA, MaximaTM SYBR Green Master Mix (Thermo Fisher), and 0.3 μM primers using a CFX Opus 384 system (Bio-Rad). ..

    Polymerase Chain Reaction:

    Article Title: Extracellular vesicles carrying surface-anchored adiponectin prevent obesity-related metabolic complications by enhancing insulin sensitivity
    Article Snippet: Liver and muscle RNA were extracted using the Maxwell® RSC simplyRNA Tissue Kit and instrument (Promega). .. RNA quantity and purity were assessed via NanoDrop ND-2000 (Thermo Fisher). cDNA was synthesized from 1 μg RNA using SuperScriptTM II (Invitrogen) and random hexamers, then purified with the Qiaquick PCR kit (Qiagen). qPCR was performed with 3 ng cDNA, MaximaTM SYBR Green Master Mix (Thermo Fisher), and 0.3 μM primers using a CFX Opus 384 system (Bio-Rad). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Extracellular vesicles carrying surface-anchored adiponectin prevent obesity-related metabolic complications by enhancing insulin sensitivity
    Article Snippet: Liver and muscle RNA were extracted using the Maxwell® RSC simplyRNA Tissue Kit and instrument (Promega). .. RNA quantity and purity were assessed via NanoDrop ND-2000 (Thermo Fisher). cDNA was synthesized from 1 μg RNA using SuperScriptTM II (Invitrogen) and random hexamers, then purified with the Qiaquick PCR kit (Qiagen). qPCR was performed with 3 ng cDNA, MaximaTM SYBR Green Master Mix (Thermo Fisher), and 0.3 μM primers using a CFX Opus 384 system (Bio-Rad). ..

    Article Title: Stress-induced vtRNA1-1 modulates redox homeostasis and ferroptosis susceptibility in hepatocellular carcinoma cells
    Article Snippet: Total RNA was extracted from the harvested cells using TRI-reagent (Zymo Research, R2050-1-200) according to the manufacturer’s recommendations. .. Reverse transcription of total RNA was performed using SuperScript IV One-Step RT-PCR System (Invitrogen, 18090010), and random hexamers following the manufacturer’s recommendations. qPCR reactions were performed using Rotor Gene-Q (QIAGEN), and analyses were conducted in Robotics software (QIAGEN) according to the manufacturer’s instructions. .. For northern blot, 4-6 μg of extracted total RNA was separated on a denaturing polyacrylamide gel (7 M Urea, 1x TBE buffer), transferred to a nylon membrane (Amersham Hybond N+; GE Healthcare, RPN203B), UV-crosslinked, and probed with 5′- 32 P-end labeled antisense DNA probes as described in Gebetsberger et al. [ ].

    SYBR Green Assay:

    Article Title: Extracellular vesicles carrying surface-anchored adiponectin prevent obesity-related metabolic complications by enhancing insulin sensitivity
    Article Snippet: Liver and muscle RNA were extracted using the Maxwell® RSC simplyRNA Tissue Kit and instrument (Promega). .. RNA quantity and purity were assessed via NanoDrop ND-2000 (Thermo Fisher). cDNA was synthesized from 1 μg RNA using SuperScriptTM II (Invitrogen) and random hexamers, then purified with the Qiaquick PCR kit (Qiagen). qPCR was performed with 3 ng cDNA, MaximaTM SYBR Green Master Mix (Thermo Fisher), and 0.3 μM primers using a CFX Opus 384 system (Bio-Rad). ..

    Reverse Transcription:

    Article Title: Mitochondrial damage drives T-cell immunometabolic paralysis after major surgery.
    Article Snippet: Quantity and purity of RNA were assessed on a NanoDrop2000 spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA was synthesized with equal amounts of RNA using a Superscript III reverse transcriptase (Invitrogen), oligo (dT) primers and random hexamers (Qiagen). ..

    Article Title: Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System.
    Article Snippet: .. RT was performed using two RT enzymes (SSIV and RevertAid Reverse Transcriptase) and random hexamers, as well as with one-step RT-PCR (QIAGEN OneStep RT-PCR Kit) and the Rev-mix1 oligonucleotide set. ..

    Article Title: Sex Differences in B2 SINE RNA Expression and Their Role in Hippocampal Development.
    Article Snippet: .. Residual gDNA was removed, and then, cDNA was prepared using random hexamers using the QuantiTect Reverse Transcription kit according to the manufacturer’s protocol (Qiagen). ..

    Article Title: Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System
    Article Snippet: .. RT was performed using two RT enzymes (SSIV and RevertAid Reverse Transcriptase) and random hexamers, as well as with one-step RT-PCR (QIAGEN OneStep RT-PCR Kit) and the Rev-mix1 oligonucleotide set. ..

    Article Title: Stress-induced vtRNA1-1 modulates redox homeostasis and ferroptosis susceptibility in hepatocellular carcinoma cells
    Article Snippet: Total RNA was extracted from the harvested cells using TRI-reagent (Zymo Research, R2050-1-200) according to the manufacturer’s recommendations. .. Reverse transcription of total RNA was performed using SuperScript IV One-Step RT-PCR System (Invitrogen, 18090010), and random hexamers following the manufacturer’s recommendations. qPCR reactions were performed using Rotor Gene-Q (QIAGEN), and analyses were conducted in Robotics software (QIAGEN) according to the manufacturer’s instructions. .. For northern blot, 4-6 μg of extracted total RNA was separated on a denaturing polyacrylamide gel (7 M Urea, 1x TBE buffer), transferred to a nylon membrane (Amersham Hybond N+; GE Healthcare, RPN203B), UV-crosslinked, and probed with 5′- 32 P-end labeled antisense DNA probes as described in Gebetsberger et al. [ ].

    Article Title: Sex Differences in B2 SINE RNA Expression and its Role in Hippocampal Development
    Article Snippet: .. Residual gDNA was removed and cDNA then prepared using random hexamers with the QuantiTect Reverse Transcription kit according to the manufacturer’s protocol (Qiagen). ..

    Article Title: ORP5 controls the partitioning of phosphatidic acid between triacylglycerol and cardiolipin synthesis at mitochondria–ER–lipid droplet contact sites
    Article Snippet: Total RNA was extracted from 3T3-L1 cells using the RNeasy mini kit (Qiagen, Valencia, CA). .. Complementary DNA was generated from 1 μg of RNA using random hexamers and the QuantiTect Reverse Transcription Kit (Quiagen, #205311) following manufacturer’s instructions. .. Quantitative real-time PCR was performed using LightCycler ® 480 SYBR Green I Master (Roche, #04707516001) in an LightCycler ® 96 Instrument Real-Time System (Roche).

    One Step RT-PCR:

    Article Title: Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System.
    Article Snippet: .. RT was performed using two RT enzymes (SSIV and RevertAid Reverse Transcriptase) and random hexamers, as well as with one-step RT-PCR (QIAGEN OneStep RT-PCR Kit) and the Rev-mix1 oligonucleotide set. ..

    Article Title: Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System
    Article Snippet: .. RT was performed using two RT enzymes (SSIV and RevertAid Reverse Transcriptase) and random hexamers, as well as with one-step RT-PCR (QIAGEN OneStep RT-PCR Kit) and the Rev-mix1 oligonucleotide set. ..

    Article Title: Stress-induced vtRNA1-1 modulates redox homeostasis and ferroptosis susceptibility in hepatocellular carcinoma cells
    Article Snippet: Total RNA was extracted from the harvested cells using TRI-reagent (Zymo Research, R2050-1-200) according to the manufacturer’s recommendations. .. Reverse transcription of total RNA was performed using SuperScript IV One-Step RT-PCR System (Invitrogen, 18090010), and random hexamers following the manufacturer’s recommendations. qPCR reactions were performed using Rotor Gene-Q (QIAGEN), and analyses were conducted in Robotics software (QIAGEN) according to the manufacturer’s instructions. .. For northern blot, 4-6 μg of extracted total RNA was separated on a denaturing polyacrylamide gel (7 M Urea, 1x TBE buffer), transferred to a nylon membrane (Amersham Hybond N+; GE Healthcare, RPN203B), UV-crosslinked, and probed with 5′- 32 P-end labeled antisense DNA probes as described in Gebetsberger et al. [ ].

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System.
    Article Snippet: .. RT was performed using two RT enzymes (SSIV and RevertAid Reverse Transcriptase) and random hexamers, as well as with one-step RT-PCR (QIAGEN OneStep RT-PCR Kit) and the Rev-mix1 oligonucleotide set. ..

    Article Title: Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System
    Article Snippet: .. RT was performed using two RT enzymes (SSIV and RevertAid Reverse Transcriptase) and random hexamers, as well as with one-step RT-PCR (QIAGEN OneStep RT-PCR Kit) and the Rev-mix1 oligonucleotide set. ..

    Software:

    Article Title: Stress-induced vtRNA1-1 modulates redox homeostasis and ferroptosis susceptibility in hepatocellular carcinoma cells
    Article Snippet: Total RNA was extracted from the harvested cells using TRI-reagent (Zymo Research, R2050-1-200) according to the manufacturer’s recommendations. .. Reverse transcription of total RNA was performed using SuperScript IV One-Step RT-PCR System (Invitrogen, 18090010), and random hexamers following the manufacturer’s recommendations. qPCR reactions were performed using Rotor Gene-Q (QIAGEN), and analyses were conducted in Robotics software (QIAGEN) according to the manufacturer’s instructions. .. For northern blot, 4-6 μg of extracted total RNA was separated on a denaturing polyacrylamide gel (7 M Urea, 1x TBE buffer), transferred to a nylon membrane (Amersham Hybond N+; GE Healthcare, RPN203B), UV-crosslinked, and probed with 5′- 32 P-end labeled antisense DNA probes as described in Gebetsberger et al. [ ].

    Generated:

    Article Title: ORP5 controls the partitioning of phosphatidic acid between triacylglycerol and cardiolipin synthesis at mitochondria–ER–lipid droplet contact sites
    Article Snippet: Total RNA was extracted from 3T3-L1 cells using the RNeasy mini kit (Qiagen, Valencia, CA). .. Complementary DNA was generated from 1 μg of RNA using random hexamers and the QuantiTect Reverse Transcription Kit (Quiagen, #205311) following manufacturer’s instructions. .. Quantitative real-time PCR was performed using LightCycler ® 480 SYBR Green I Master (Roche, #04707516001) in an LightCycler ® 96 Instrument Real-Time System (Roche).



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